Dorsal lip of blastopore3/21/2024 ![]() a = anterior an = animal bc = blastocoel bp = blastopore d = dorsal dl = dorsal lip dm = deep mesoderm fg = foregut fp = floor plate hg = hindgut l = left no = notochord ov = otic vesicle p = posterior r = right s = somite v = ventral veg = vegetal. (E’,E”) Histological sections (levels indicated in (E)) highlighting expression in the otic vesicle (E’) and nephrostomes ((E”), arrowheads). (E) Staining in the otic vesicle and nephrostomes (arrowheads). Our results suggest that the dorsal blastopore lip (DBL) at the posterior end of the embryo is the source of the PCP cue. Enlargements show multiciliated skin cells ( (D”) cilia indicated by arrowheads) and GRP cells after intercalation into the notochord ( (D”’) arrowheads). (D) Expression at stage 23 in ciliated cells of the epidermis and the floor plate (sagittal section shown in (D’)). (C’) Histological section (plane marked in (C)). In an early gastrula, mesoderm (red) involution occurs at the dorsal lip of the blastopore. (C) Expression in the gastrocoel roof plate (GRP) at stage 17 (dorsal explant shown in ventral view). Figure 5: Movement at the lip of the blastopore (dorsal view). Note that the superficial mesoderm (SM) was free of PACRG mRNA (inset in (B’)). (B’) Sagittal hemisection (plane indicated in (B)) revealing mRNA localization in deep mesoderm. Persistent differences in staining intensities between dorsal and ventral side. Enlargements (boxes) indicate higher expression levels on the dorsal side. ![]() (A’) Sagittal hemisection of embryo shown in (A). (A) Expression at the four-cell stage (top view). PACRG expression during early Xenopus development.
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